ELECTRON MICROSCOPE OBSERVATIONS ON MYOSIN FROM PHYSARUM POLYCEPHALUM
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چکیده
منابع مشابه
Electron Microscope Observations on Myosin from Physarum Polycephalum
Myosin has been separated from Physarum polycephalum actomyosin in confirmation of the results of Hatano and Tazawa. In an intermediate step, myosin-enriched actomyosin has also been obtained. The mean yield of free myosin was 4.4 mg from 100 g of mold. It was obtained as water-clear solutions at micro = 0.055 with calcium ATPase activity of up to 0.5 microM P(i)/min per mg. Negatively stained ...
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Actin-myosin-ATP interaction is supposed to produce the motive force for cytoplasmic streaming in plasmodia of Physarum polycephalum.' We found that the actin-myosin-ATP interaction as measured by superprecipitation of myosin B (natural actomyosin) was inhibited by Ca2+.2) As half-maximal inhibition occurred at ,uM levels of Ca2+, we proposed that Ca2+ controls cytoplasmic streaming by inhibiti...
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Physarumpolycephalum is an acellular slime mould whose histones are of general interest because they allow comparisons to be made between lower eukaryote and higher eukaryote histones and, perhaps more importantly, they provide a unique opportunity for the study of histone changes in the mitotic cycle, which is naturally synchronous in P. polycephalum plasmodia, and during differentiation. Stud...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1972
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.52.3.648